integration site meaning in Chinese
整合位点,整合部位
Examples
- The mechanisms of gene silencing include copy numbers and configuration of transgene , integration sites in plant and transcription of transgene , etc
基因沉默的机制是多方面的，包括转基因的拷贝数和构型、在植物上的整合位点、转基因的转录水平等。 - To abolish transcription efficiency affected by copy number or integration site in chromosome , all the vectors were integrated into frt site by co - transfecting with an flp recombinase producing plasmid
应用w七stemblot对其中的20个克隆进行检测，选出一个caspase一3表达量最低的细胞克隆a6 。 - In abroad , the study of integration site used for transgenic detection had just begun . in this study , according to the collection of the global commercialized transgenic crops , select seven exogenous genes which basically cover the total commercialized crops , namely camv35s and fmv promoter , nos terminater , mark gene nptii , and aim genes pat , epsps and cryia ( b ) . use endogenous 18srrna gene as collate , design a large pairs of specific primers , screen the optimum primers groups , optimized the test condition and parameters , establishing the qualitative pcr detection system
本研究根据收集的国内外已商品化的转基因作物品种，选择了能基本覆盖商品化转基因品种的7个外源基因，即： camv35s 、 fmv启动子、 nos终止子、 npt标记基因和目的基因pat 、 epsps 、 cryia ( b )作为筛选目标，以植物18srrna基因作为内源参照基因，设计了多对特异性引物，并筛选出最佳组合，优化了检测条件和参数，建立了pcr定性检测方法体系。 - Transgenic integration site detection is the efficient method to identify whether the transgenic crops are approved to import or export . some quantitative detection methods were reported in 2000 and 2001 , and that is about bt11 and mon810 corn . real - time 5 " - nuclease pcr had been previously used successfully to determine quantitatively roundup ready ( rr ) soy and btl76 maize in food
转基因整合位点检测是鉴定转基因品种是否为批准进口转基因作物的有效的特异性方法，国外在2000年和2001年已开展了转基因玉米bt11 、 mon810两个品种整合位点的特异性检测方法研究。 - The env protein deduced from env gene encodes the hydrophilic surface protein ( su ) and the hydrophobic transmembrane domain ( tm ) that determine the specific interaction between virus particles and cell surface receptors during retroviral entry . the su of retroviruses is a highly variable genetic element , containing receptor binding sites and major antigenic determinants . exjsrv - specific dna probes were derived . by using these dna probes in tissue hybridization . we successfully identified jsrv mrna expression and proviruses dna in sheep lung tissues infected with jsrv and control group has no postive signals , validating the use of exogenous virus - specific dna probes in the analysis of oncogenic proviral integration sites and identification of integrated exogenous proviral sequences
用地高辛随机引物法标记exjsrv特异的env片段，制备探针，原位杂交检测spa肺组织中的rna及前病毒dna ，结果表明spa患羊肺组织内有jsrvenv基因mrna的表达，同时也检测到了前病毒dna ，而相应的阴性对照却无阳性信号，证实外源性病毒特异的dna探针在致瘤性前病毒的整合位点和整合的外源性前病毒的检测中具有可信度。